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A Bioreactor Method to Generate High-titer, Genetically .

Nov 05, 2017 · Here we report a bioreactor method based on continuous infection, where retinal pigment epithelial (ARPE-19) cells adhered to microcarrier beads are infected in a bioreactor and used to produce high-titers of clinical isolate HCMV that maintain genetic integrity of key viral tropism factors and the viral genome.

Utilizing Bioreactors to Increase Virus Production in .

May 23, 2012 · More cells in culture increases overall titer yield. In addition, the use of microcarriers allow manufacturers to increase the number of cells that can be cultured in one tank enabling more efficient large-scale production and permitting the use of greater than 1,000 liter bioreactors.

Perfusion Cell Culture :: Repligen

Apr 01, 2015 · Separate advances in cell line engineering, media composition, and bioreactor design generated magnitude-fold increases in titers for batch and fed-batch modes, thereby reducing the need for adoption of perfusion technology during that time. Recently, however, the need for alternative manufacturing strategies that can boost efficiency and .

Comparison of Different Bioreactor Systems for the .

The performance evaluation results using mathematical models indicated that the fixed bed bioreactor has a higher potential for retroviral vector production because of both the higher reactor productivity and the lower sensitivity of productivity in relation to the changes in final retrovirus titer in the range of 3 × 10 6 to 15 × 10 6 PFU cm .

BIOSTAT® Cplus - Stainless Steel Fermenter / Bioreactor .

The Stainless Steel Fermenter | Bioreactor for Your Laboratory. The Biostat® Cplus is a Sterilizable-In-Place (SIP) Fermenter | Bioreactor developed for the cultivation of microorganisms and cell cultures. Culture vessels with working volumes of 5 L, 10 L, 15 L, 20 L and 30 L are available.

Lentiviral vector production for cell and gene therapy .

different volumes ranging from a 96 deep-well plate to a 2 L bioreactor using LV-MAX Lentiviral Production System. The lentiviral titer was determined by transducing HT1080 cells and analyzing GFP-positive cells; similar titers .

Bioprocess Monitoring of Antibody Titer

Utilize protein titer in real time to extract maximum value from every bioreactor run, with Tridex ™ from IDEX Health & Science. Engineered for convenience, Tridex allows you to achieve accurate titer measurements, which correlate to off-line HPLC, in only five minutes.

(PDF) Continuous influenza virus production in a tubular .

Nov 05, 2019 · titers in the tubular bioreactor harvest were below the detection limit, and the TCID. 50. titers corre-sponded to the VS. This indicated that the .

Continuous Cell Culture Operation at 2,000-L Scale

titers from fed-batch cell cultures. One consequence has been a move toward smaller bioreactors and replacement of older stainless steel equipment with single-use technologies. Some companies are choosing to develop perfusion processes. They are especially useful when a therapeutic product is labile and has a short halflife within a

Scalable, high performance single-use bioreactor .

Minor process optimization led to similar titers in the scale-X bioreactor. Process scale-up and chained TFF concentration. Experimental results were extrapolated for large scale production, both for the reference process and optimized . process (Figure 7). Experimental volumes of the scale-X hydro bioreactor (2.4m. 2) are extrapolated to scale .

scale-X™ bioreactor for enhanced upstream processing .

The scale-X technology was designed to achieve cost-effective large capacity production. Integrated into the NevoLine™ biomanufacturing platform, the scale-X nitro bioreactor enables the future of large-scale viral production.. The scale-X hydro and carbo systems offer scale-down models suited to meet smaller production demands.

Production Processes for Monoclonal Antibodies | IntechOpen

The first single-use bioreactors emerged in the late 1990s with the launch of a wave reactor system. After that, disposable stirred tank bioreactors were developed . This method brought many advantages for mAb manufacturing. At the end of the process, the bioreactor .

Fed-Batch Cell Culture Process Development: Implementing a .

Sep 11, 2014 · Figure 4 shows growth and titer profiles that resulted under temperature and no temperature shift. As a result of the temperature shift, peak VCD was lower but the cell culture duration was prolonged to 17 days, so titers increased to 4.4 g/L. We also ran project 5 at the 200-L and 1,000-L scales, which also achieved titers >3.0 g/L (Table 3).

Single-use Bioreactors Have Reached the Big Time .

Mar 01, 2017 · As such, Norris sees single-use bioreactor processes designed with the entire workflow in mind, delivering a high quality, optimal biologic titer into the downstream unit operation. Adds Adams: "single-use bioreactor technology has matured to the point where suppliers now offer large-scale, robust, well-characterized products that are .

30 Years of Upstream Titer Productivity .

Titer is the amount (mass, measured as weight) of an expressed agent — generally a protein in aqueous solution — relative to the volume of total upstream-produced liquid containing the agent (with the bioreactor volume often used for this amount). Data are provided in terms of grams per liter (g/L). Titer is the primary benchmark characterizing upstream manufacturing efficiency, with .

Development of a high-yield live-virus vaccine production .

Apr 29, 2020 · The stock VSVΔG/LASVGP, stock titer of 4.9 × 10 8 pfu/mL, was used for all infection studies utilizing the bioreactor. Infection of the bioreactor with virus inoculum was performed five days post-seeding or when peak cell density was obtained, evident by .

scale-X™ bioreactor for enhanced upstream processing .

The scale-X technology was designed to achieve cost-effective large capacity production. Integrated into the NevoLine™ biomanufacturing platform, the scale-X nitro bioreactor enables the future of large-scale viral production.. The scale-X hydro and carbo systems offer scale-down models suited to meet smaller production demands.

Transient Lentiviral Vector Production in HEK 293T Cells .

production method, the unconcentrated titers and physical titers were lower for the packed-bed bioreactor approach with 3.5 x 104 TU/mL vs. 4.5 x 105 TU/mL, and 1.0 x 107 VP/mL vs. 1.2 x 108 VP/mL, respectively. The lower cell concentration at the time of transfection in the bioreactor approach could be one explanation for the lower yield.

Markets Expand for Single-Use Bioreactors

According to Transparency Market Research, the global market for single-use bioreactors will increase 11% per year between 2015 to 2024, from 2.4 billion to 5.9 billion.

EfficientFeed™ A+ AGT™ Supplement

Flexibility to further increase titers are enhanced with the ability to super-concentrate the product by reconstituting with less water to deliver up to 199 g/L of fed nutrients (see figure). Maximizing Bioreactor Utilization

Production Processes for Monoclonal Antibodies | IntechOpen

The first single-use bioreactors emerged in the late 1990s with the launch of a wave reactor system. After that, disposable stirred tank bioreactors were developed . This method brought many advantages for mAb manufacturing. At the end of the process, the bioreactor is discarded and replaced by a new clean and sterile one.

High Titers and Perfusion Processes Challenge Cell .

Sep 01, 2015 · SCIEPRO/Getty Images Cell harvesting is a crucial step in biopharmaceutical manufacturing that can have significant impacts on product quality and the design of remaining downstream processes. Growing titers and viable cell densities, due to improved media and cell lines, and growing use of perfusion cell culture are making this downstream purification step more challenging.

Hollow fiber bioreactor - Wikipedia

High titers were achieved for both the suspension and adherent strains; furthermore, the hollow fiber bioreactor technology was found comparable in its production capacity to that of other commercial bioreactors on the market, including classic stirred-tank and wave bioreactors (Wave) and ATF perfusion systems.

High density Huh7.5 cell hollow fiber bioreactor culture .

Nov 30, 2018 · In the bioreactor used here, peak HCV infectivity titers of up to 7.6 log 10 FFU/mL were achieved using a JFH1-based recombinant with genotype 5a specific Core-NS2.

Continuous influenza virus production in a tubular .

Continuous tubular bioreactor system: Set-up and operation. A continuous tubular bioreactor system consisting of a 500 mL CSTR followed by a 211 mL PFBR with a nominal production capacity of 12 mL/h was established as shown in Fig 1, and the system was operated in a cultivation room at a controlled and stable temperature of 37°C.The first bioreactor unit .

Virus harvesting in perfusion culture: Choosing the right .

Jun 22, 2020 · The increased cell concentration allows for higher virus titers. To retain cells in the bioreactor vessel, cell retention devices are required that are typically classified by their physical .

(PDF) Transient Lentiviral Vector Production Using a .

As a result, cumulative functional LV titers were increased by up to 15-fold compared to batch mode, reaching a cumulative total yield of 8 x 1010 TU/L of bioreactor culture.

Cell culture processes for monoclonal antibody production

opment work in cell line, media and bioreactor condition opti-mization, cell specific productivity of over 20 pg/cell/day can be routinely achieved for production cell lines; 1. high titers up to ∼10 g/L and cell densities of over 20 million cells/mL in fed-batch processes have been recently reported by a few companies at major conferences.

PEI-Mediated Transient Transfection of High Five Cells at .

18 hours ago · strategy at bioreactor scale was demonstrated, with no di erences in terms of transfection e ciency and a 1.8-fold increase in VLP titer at 72 hpt in comparison to the optimized conditions in shake flasks. The size of Gag-eGFP VLPs obtained corresponds to that observed in VLPs produced with the reference system based on the BEVS.

The Biomanufacturing Blog

High-Throughput DoE Of 52 Parallel Bioreactors Doubles Titer In 1 Week. Data from an ongoing collaboration with Cytovance Biologics to rapidly optimize and scale. March 03. Biomanufacturing. Culture's Founding Story: An Interview with CEO Will Patrick.