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Microsystems for biofilm characterization and sensing – A .

The CDC biofilm reactor (CBR) allows for the growth of biofilms under moderate to high fluid shear stress . The reactor, shown in Fig. 1c, incorporates 24 removable biofilm growth surfaces (coupons) for sampling and analysis, and consists of a 1l glass vessel with an outlet positioned to provide about 350 ml of working fluid capacity. The .

α-Chymotrypsin Immobilized on a Low-Density Polyethylene .

Dec 12, 2018 · E. coli biofilm was grown on non-functionalized (LDPE and LDPE-GA) and functionalized (LDPE-α-CT) coupons in the CDC biofilm reactor (Biosurface Technologies, Bozeman, MT, USA) according to Cattò et al. . Briefly, the bioreactor was inoculated with 400 mL of sterile LB medium with the addition of 1 mL of diluted pre-washed overnight culture .

Biofilm removal by medical device cleaners: comparison of .

Feb 01, 2010 · The CDC bioreactor enables the repeatable growth of bacteria on 24 removable discs. 10 In this study we have validated and refined the use of biofilm-covered discs to test biofilm removal performance as devised by Charlton. 11 In Charlton's test, coupons were individually removed from the coupon holder and suspended on clips for cleaning and .

Influence of feed temperature to biofouling of .

Jun 01, 2019 · Each cleaning step was performed for 30 min at 50 °C. A rinsing step with deionised water at 50 °C preceded and followed each individual step of the CIP. The stirring rate of 180 rpm was maintained in the biofilm reactor during the cleaning procedure. A cleaning step under the same conditions was also performed at the end of each experiment. 2.4.

Publications | World Academy of Science, Engineering and .

Authors: Nor'Aini Yusof, Kong-Seng, Lai Abstract: Organizational culture fosters innovation, and innovation is the main engine to be sustained within the uncertainty market. Like other countries, the construction industry significantly contributes to the economy, society and technology of Malaysia, yet, innovation is still considered slow compared to other industries such as manufacturing.

(PDF) A Modified CDC Biofilm Reactor to Produce Mature .

worst case scenario, amputation. . suggesting that when grown on a complex substrate, biofilms may be more tolerant to antibiotics. . The CDC biofilm reactor is a robust culture system with .

Full text of "Disinfection And Decontamination Principles .

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Environmental Science & Technology | Vol 52, No 22

Effects of Chloramine and Coupon Material on Biofilm Abundance and Community Composition in Bench-Scale Simulated Water Distribution Systems and Comparison with Full-Scale Water Mains. Srijan Aggarwal*, C. Kimloi Gomez-Smith, Youchul Jeon, Timothy M. LaPara, Michael B. Waak, and ; .

2012BIOMATTER0004R2 | Biofilm | Staphylococcus Aureus

(4) Finally, of utmost importance is the interpretation of results and evaluation of assay quality.170 In the study conducted by Extremina et al., the use of BFi confirmed that E1162Desp isolate is a good negative control for biofilm quantification and demonstrates the importance of knowing the growth index of different sets of strains or .

Removing Biological and Chemical Contamination from a .

The first configuration used was the Centers for Disease Control and Prevention (CDC) biofilm reactors (obtained from BioSurface Technologies®, Bozeman, MT). The second configuration was a pipe section reactor constructed in a lab that was operated as a single flow through with creeping flow (1 mL/min, Reynolds number [Re] 1). The third .

Biofilm removal by medical device cleaners: comparison of .

Biofilms grown in these conditions using a CDC bioreactor were very different from the biofilms formed at the bottom of a well and had a much more complex and robust structure.

Full text of "Disinfection And Decontamination Principles .

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Biofouling of Polyamide Membranes: Fouling Mechanisms .

Aug 30, 2019 · Cleaning later than recommended has been shown to result in more frequent cleanings overall and subsequent shorter membrane life due to membrane damage that occurs under cleaning conditions [243,246,247,248]. Membrane cleaning involves chemical and physical interactions in an attempt to remove accumulated biofouling. Chemical reaction between .

Worst-case biofilm conditions for cleaning evaluations

Oct 04, 2016 · Worst-case biofilm conditions for cleaning evaluations . 4-Oct-2016 . Cleaning. A recent study carried out by R. Bright, A. Deal, D. Klein and P. Lopolito at the Life Sciences Division of STERIS highlights the importance of testing against both typical and atypical biofilms when evaluating cleaning and disinfection chemistries for biofilm prevention on process equipment

Biofouling of Polyamide Membranes: Fouling Mechanisms .

Aug 30, 2019 · Cleaning later than recommended has been shown to result in more frequent cleanings overall and subsequent shorter membrane life due to membrane damage that occurs under cleaning conditions [243,246,247,248]. Membrane cleaning involves chemical and physical interactions in an attempt to remove accumulated biofouling. Chemical reaction between .

Is peracetic acid suitable for the cleaning step of .

Sep 16, 2014 · Core tip: Some formulations based on peracetic acid (PAA) are recommended by manufacturers for cleaning flexible endoscopes. We reviewed 243 studies to analyse the evidence for this recommendation. No study demonstrated that PAA-based cleaners were as effective as conventional cleaners, and some PAA-based formulations had clear biofilm-fixation potential.

2012BIOMATTER0004R2 | Biofilm | Staphylococcus Aureus

(4) Finally, of utmost importance is the interpretation of results and evaluation of assay quality.170 In the study conducted by Extremina et al., the use of BFi confirmed that E1162Desp isolate is a good negative control for biofilm quantification and demonstrates the importance of knowing the growth index of different sets of strains or .

METHODS AND COMPOSITIONS FOR CLEANING AND . - Justia

Mar 14, 2014 · This application relates to methods and compositions for cleaning and disinfecting unclean surfaces that are contaminated, typically with bacteria, viruses, yeast and molds. Broadly speaking contaminated surfaces includes hard and soft surfaces such as those found in environments, in industrial environments, and hospitals, as well as surfaces of food products such as .

Biofilm Formation in Milk Production and Processing .

Biofilm development is a result of successful attachment and subsequent growth of microorganisms on a surface . Under suitable conditions, a biofilm in a milk processing environment develops initially through accumulation of organic matter on a metal surface, which is then colonized by bacteria.

Adhesion of Pathogenic Bacteria to Food Contact Surfaces .

Oct 11, 2010 · 1. Introduction. In food processing plants, residues of all kinds chemical, biological, organic, or inorganic inevitably accumulate on the surfaces of equipments in contact with food [].Attachment of undesirable microorganisms to these surfaces is a source of concern, since this can result in product contamination leading to serious economic and health problems [2–4].

A method for rapid quantitative assessment of biofilms .

Biofilm culture. Sample coupons were exposed to a liquid culture of Pseudomonas putida (ATCC 39169) bacteria, then stained, photographed, and analyzed using an image analysis algorithm program written for the purpose. In parallel, identical coupons were analyzed by a standard method: cell density of the biofilm was measured after removing the cells by sonication and dispersing them in solution.

Waterborne microorganisms and biofilms related to hospital .

Water is the main stimulus for the development of microorganisms, and its flow has an important role in the spreading of contaminants. In hospitals, the water distribution system requires special attention since it can be a source of pathogens, including those in the form of biofilms often correlated with resistance of microorganisms to various treatments.

Production and analysis of a Bacillus subtilis biofilm .

May 01, 2018 · Bacillus subtilis is a spore-forming soil bacterium that is capable of producing robust biofilms. Sporulation can occur in B. subtilis biofilms and it is possible that the spores embedded in the protective matrix could present a significant challenge to disinfecting agents or processes. This article describes a method for the growth and quantification of a reproducible B. subtilis ATCC 35021 .

Characterization of Biofilms on Medical Device Materials .

drip flow reactor. The purpose of this study was to mimic relevant biofilm growth conditions and modify a colorimetric assay for quantifying biofilm accumulation. First, biofilms were grown under both static and dynamic conditions on coupons representative of four commonly used medical device materials: 316L stainless steel, polycarbonate,

(PDF) Growth substrate may influence biofilm .

Mar 14, 2019 · The CDC biofilm reactor is a robust culture system with high reproducibility in which biofilms can be grown for a wide variety of analyses. Multiple material types are available as growth .

Removing Biological and Chemical Contamination from a .

The first configuration used was the Centers for Disease Control and Prevention (CDC) biofilm reactors (obtained from BioSurface Technologies®, Bozeman, MT). The second configuration was a pipe section reactor constructed in a lab that was operated as a single flow through with creeping flow (1 mL/min, Reynolds number [Re] 1). The third .

International Ultraviolet Association Inc - 2019 World .

P. aeruginosa was chosen as a target microorganism and was grown in a CDC reactor on clear polycarbonate coupons. Coupons were treated with UV light at 265 nm using a UV LED collimator beam. Manufacturer-recommended wiping techniques were compared to more realistic wiping regimes and to UV LED doses ranging from 2-20 mJ/cm2.

The Use of Drip Flow and Rotating Disk Reactors for .

The drip flow reactor is ideal for microsensor monitoring, general biofilm studies, biofilm cryosectioning samples, high biomass production, medical material evaluations, and indwelling medical .

acetate | SPE

However, in recent years, various other micro-organisms are believed to be involved in souring, e.g. Sulfate reducing archaea (SRA). In this study, fixed film up flow bioreactors (FFUBR) were utilized to assess the potential for H 2 S production or changes in such H 2 S production, when seawater is injected into a North Sea oil reservoir. The .

Frontiers | Eradication of high viable loads of Listeria .

This study demonstrates the efficacy of cold gaseous ozone treatments at low concentrations in the eradication of high Listeria monocytogenes viable cell loads from glass, polypropylene, stainless steel, and expanded polystyrene food-contact surfaces. Using a step by step approach, involving the selection of the most resistant strain-surface combinations, 11 Listeria sp. strains resulted .